Whole-Genome Sequencing of a Single Viral Species from a Highly Heterogeneous Sample

Citation:

Han, H. - S. ; Cantalupo, P. G. ; Rotem, A. ; Cockrell, S. K. ; Carbonnaux, M. ; Pipas, J. M. ; Weitz, D. A. Whole-Genome Sequencing of a Single Viral Species from a Highly Heterogeneous Sample. Angewandte Chemie 2015, 54, 13985-13988. Copy at http://www.tinyurl.com/y5f36zqx
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Abstract:

Metagenomic studies suggest that only a small fraction of the viruses that exist in nature have been identified and studied. Characterization of unknown viral genomes is hindered by the many genomes populating any virus sample. A new method is reported that integrates drop‐based microfluidics and computational analysis to enable the purification of any single viral species from a complex mixed virus sample and the retrieval of complete genome sequences. By using this platform, the genome sequence of a 5243 bp dsDNA virus that was spiked into wastewater was retrieved with greater than 96 % sequence coverage and more than 99.8 % sequence identity. This method holds great potential for virus discovery since it allows enrichment and sequencing of previously undescribed viruses as well as known viruses.

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Last updated on 11/12/2020