A high-throughput cellulase screening system based on droplet microfluidics

Citation:

Ostafe, R. ; Prodanovic, R. ; Ung, W. L. ; Weitz, D. A. ; Fischer, R. A high-throughput cellulase screening system based on droplet microfluidics. Biomicrofluidics 2014, 8 041102. Copy at http://www.tinyurl.com/ldmya93
ostafe2014.pdf1.3 MB

Abstract:

A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells.

Publisher's Version

Last updated on 11/13/2020