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Massively parallel single-nucleus RNA-seq with DroNc-seq

Citation:

Habib, N. ; Avraham-Davidi, I. ; Basu, A. ; Burks, T. ; Shekhar, K. ; Hofree, M. ; Choudhury, S. R. ; Aguet, F. ; Gelfand, E. ; Ardlie, K. ; et al. Massively parallel single-nucleus RNA-seq with DroNc-seq. Nat. Methods 2017, 14, 955. Copy at http://www.tinyurl.com/y2cyr78g
habib2017.pdf5.44 MB

Date Published:

Aug

Abstract:

Single-nucleus RNA sequencing (sNuc-seq) profiles RNA from tissues that are preserved or cannot be dissociated, but it does not provide high throughput. Here, we develop DroNc-seq: massively parallel sNuc-seq with droplet technology. We profile 39,111 nuclei from mouse and human archived brain samples to demonstrate sensitive, efficient, and unbiased classification of cell types, paving the way for systematic charting of cell atlases.

Publisher's Version

Last updated on 11/09/2020